Subject: scale up
Date: 1992-05-19 17:09:18 GMT
OFFICE MEMO scale up
js writes>> all the extra stages. I can't believe the yeast cares
whether it is in a
>> gallon of wort or an ounce.
bb replies>I don't agreee with this statement...i.e., If you dilute
>yeast too much, they seem to slow down
>more than proportionately.
While on this topic, I thought I'd bring up my experience. First, I
bring up active
cultures from single colonies (see my "too-scientific" yeast primer
from a few issues
ago). I have found that it is best to scale up gradually (ie. single
colony --> small
culture--> large culture, etc.). The reason is purely from the
avoiding contamination. If you inoculate a medium with 1% active cells,
a greater chance of outcompeting anything in that other 99% than if you
with only .001%. This is a well established fact that is exploited by
run fermentations (including non-alcoholic ones). Also consider that
brewers do not sterilize, but only sanitize their wort through boiling.
will be doing your yeast a favor if you give them a numerical advantage
organisms. The bottom line: SCALING UP PRESERVES CULTURE INTEGRITY.
Hope this clears things up. Cheers!
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